mouse breast cancer cell line  (Procell Inc)

Journal: Animal Models and Experimental Medicine

Article Title: Characterizing a murine breast cancer mouse model reveals chromosomal abnormalities in structure and number of single‐cell clones and the presence of rare cancer stem cell‐like phenotypes

doi: 10.1002/ame2.70156

Figure Lengend Snippet: A 4T1 BALB/c mouse model of breast cancer. The left flanks of mice were injected with 4T1 cells subcutaneously. (A) A representative picture of an injected mouse is shown with a white arrow indicating the site of primary tumor growth. (B) The tumor volume was evaluated twice per week. (C) A representative excised tumor. (D) H&E staining of primary tumor tissue at the end point of tumor development with pleomorphic vesicular nuclei (green arrows) and hyperchrome prominent nucleoli (yellow arrows) indicated (40× magnification, and the scale bar represents 20 μm).

Article Snippet: The 4T1 mouse breast cancer cell line was purchased from the American Type Culture Collection (ATCC R CRL‐2539) (Manassas, Virginia, USA).

Techniques: Injection, Staining

Journal: Animal Models and Experimental Medicine

Article Title: Characterizing a murine breast cancer mouse model reveals chromosomal abnormalities in structure and number of single‐cell clones and the presence of rare cancer stem cell‐like phenotypes

doi: 10.1002/ame2.70156

Figure Lengend Snippet: Chromosome counts of metaphase cells. (A) Chromosome number of murine 4T1 breast cancer cells prepared ex vivo (23 cells) and in vitro (100 cells). Boxes represent data range from 1st quartile to 3rd quartile. Whiskers represent the range of chromosomes from minimum to the maximum. (B) Descriptive statistics and Mann–Whitney two‐tailed test results. (C) Distribution of chromosome numbers in ex vivo and in vitro cells.

Article Snippet: The 4T1 mouse breast cancer cell line was purchased from the American Type Culture Collection (ATCC R CRL‐2539) (Manassas, Virginia, USA).

Techniques: Ex Vivo, In Vitro, MANN-WHITNEY, Two Tailed Test

Journal: Animal Models and Experimental Medicine

Article Title: Characterizing a murine breast cancer mouse model reveals chromosomal abnormalities in structure and number of single‐cell clones and the presence of rare cancer stem cell‐like phenotypes

doi: 10.1002/ame2.70156

Figure Lengend Snippet: Analysis of breast cancer stem cell‐like phenotypes (CD44 + /CD24 − ). (A) Flow cytometric analysis of CD24/CD44 expression in ex vivo and in vitro cells. (B) Percentage of four subpopulations in breast cancer ex vivo and in vitro 4T1 cells. Standard deviation is indicated for each respective cell suspension, n = 3.

Article Snippet: The 4T1 mouse breast cancer cell line was purchased from the American Type Culture Collection (ATCC R CRL‐2539) (Manassas, Virginia, USA).

Techniques: Expressing, Ex Vivo, In Vitro, Standard Deviation, Suspension